Figure 8.

Hypoculoside but not hypoculine disrupts the membrane integrity of yeast cells. (A) Wild type yeast cells pre-treated with either DMSO or hypoculoside (5.4 μM) were exposed to FM 4-64FX. Uptake of FM 4-64FX after 0′, 30′ and 120′ was analyzed by fluorescence microscopy of fixed cells. (B) Yeast cells were incubated with either DMSO or hypoculoside or hypoculine at different concentrations (1.8, 3.5 and 5.3 µM) for 30′ and 2 hours. They were then treated with propidium iodide (PI) and percentage of PI-staining cells was assayed by fluorescence microscopy. Representative images of cells visualized by bright field and fluorescence microscopy are shown along with quantification of PI-staining cells (N = 100 cells) in (C). Representative data from three independent experiments are shown in the Figure.