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. 2019 Jan 24;9:472. doi: 10.1038/s41598-018-36778-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of hSF-MSCs. (a) Photomicrograph of calcified nodules stained by Alizarin Red indicating that hSF-MSCs had differentiated into an osteogenic cell lineage. (b) Oil Red O staining showing intracellular lipid droplets (red) in hSF-MSCs that were adipogenically-induced. (c) After chondrogenic differentiation of hSF-MSCs for 28 days, collagen type II was detected around cells by immunofluorescent staining. (d–l) Representative graphs of flow cytometry analysis of the phenotype of hSF-MSCs for MSC markers including CD44 (d), CD90 (e), CD 105 (f), and CD73 (g), and negative for CD45 (h), CD34 (i), CD11b (j), CD19 (k), and HLA-DR (l).