Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 24;9:544. doi: 10.1038/s41598-018-35811-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Impact of extracellular application of aSyn on mitochondria. Monomeric (M), oligomeric (O), or fibrillar (F) aSyn was applied to H4 cells. (a) WB analysis of the markers of mitochondrial mass (TOM20, TIM23, and HSP60). The bands for TOM20, TIM23, and β-actin (upper panel), as well as the bands for HSP60 and β-actin (lower panel) are derived from the same blots. Mitochondrial markers are normalized to β-actin of the identical blot. (b) Flow cytometric analysis of mitochondrial membrane potential-sensitive TMRE staining. Application of aSyn to cells does not change the mass of mitochondria and mitochondrial membrane potential, regardless of its aggregation state, when compared to untreated cells (Ctrl) (quantification in (a,b), n = 3, One-way ANOVA with Tukey’s multiple comparisons tests).