Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 24;9:544. doi: 10.1038/s41598-018-35811-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effects of trehalose on exogenous aSyn-induced lysosome dilation and aSyn accumulation. H4 cells (a,b) and primary neurons (c,d) were treated with trehalose prior to aSyn application (M: monomers; O: oligomers; and F: fibrils). (a,c) ICC analysis of lysosomes probed by LysoT. (b,d) The amount of larger LysoT+ clumps induced by aSyn decreases with trehalose-pretreatment. Quantification was conducted by normalizing the levels in exposed cells against untreated cells (Ctrl) (n = 3, Two-way ANOVA with Sidak’s multiple comparisons test). (e,f) ICC analysis of extracellular aSyn accumulated in fibril-exposed H4 cells (e) and primary neurons (f) with or without trehalose (+T or -T) pretreatment. Quantitative analysis of ICC images reveals that trehalose decreases the accumulation of extracellular aSyn by determining the counts of detectable aSyn signals/cell or by assessing the counts of larger aSyn aggregates (area >2 µm²)/cell (n = 3, unpaired t-test).