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. 2019 Jan 24;9:521. doi: 10.1038/s41598-018-36741-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sections of heart, muscle and pancreas of a mouse are imaged using a 20x objective of a confocal microscope, after perfusion with MHI148-PEI and clearing with ECi. (a) Thin section from heart in 3D (left). Zoomed-in view shows arteries fully stained by MHI148-PEI. A scanned depth of 65 μm was used as indicated by the depth bar with color coding (right). (b) Thin muscle section from the leg of a mouse in 3D (left). Zoomed-in view shows capillary vessels with some branches (middle). A thin section of depth of about 90 μm was scanned as shown in the depth bar with color coding (right). (c) Thin section from pancreas (left). Zoomed-in view shows vessels in pancreas. A thin section of depth of about 120 μm was scanned as shown in the depth bar with color coding (right). The colored data in 3D are the structures and the black color is used for the background in each subfigure.