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. 2019 Jan 24;9:540. doi: 10.1038/s41598-018-36695-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HTS triggers ASL secretion in vivo and ex vivo preparations. (A) scatter plot of HTS and ITS treatment on ASL height in live swine and (B) change in ASL height (HTS, n = 6 beads from 4 swine; ITS, n = 6 beads from 5 swine). (C) scatter plot of HTS and ITS treatment on ASL volume in ex vivo trachea preparation and (D) change in ASL height (HTS, n = 45 beads from 15 tracheas; ITS, n = 49 beads from 14 tracheas; control, n = 12 beads from 5 tracheas). (E) Amiloride did not affect the HTS treatment result (HTS, n = 45 beads from 15 tracheas; ITS, n = 49 beads from 14 tracheas; HTS + Amil, n = 12 beads from 5 tracheas). Data are presented as mean ± SEM and values at 18 min were analyzed with ANOVA and Tukey’s multiple comparison test. Data sets labeled with different letters differ significantly, p < 0.05.