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. 2019 Jan 24;9:487. doi: 10.1038/s41598-018-37422-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decreased QS-mediated virulence and in vivo infectivity in MDR strains. (A) Qualitative analysis of 3-oxo-C12-HSL production. An E. coli reporter strain harboring the pKDT17 plasmid was incubated with overnight-grown supernatants of each bacterial strain (indicated at the bottom) for one hour and then subjected to β-galactosidase assays. Overnight-grown culture supernatants of each strain were used for elastase (B) and pyocyanin (D) assays. (C) LasR protein sequences of PAO1, Y31, Y71, Y82, and Y89 were compared with CLUSTALW. (E) Eight-week-old BALB/C mice (n = 6) were infected with approximately 2.5 × 10⁷ bacterial cells of indicated strains. Mouse survival rates were monitored following infection. (F) After the death of mouse or 42 hours of infection, mouse lung was homogenized in PBS and serially diluted for enumerating bacterial cells. Diluted lung homogenates were spotted on Pseudomonas Isolation Agar. ***p < 0.001 vs. the CFU of the PAO1-infected samples.