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. 2019 Jan 24;9:487. doi: 10.1038/s41598-018-37422-9

Figure 5.

Figure 5

Decreased Type VI secretion system swarming activities and iron acquisition activities in MDR strains. (A) Bacterial competition assays with Vibrio cholerae V52 and P. aeruginosa. V. cholerae V52 as prey of P. aeruginosa strains, PAO1 and clinical isolates were incubated overnight in LB. V52 alone and mixtures of P. aeruginosa and V52 in a 1:1 ratio were grown on LB agar for 5 hr. Bacteria grown on LB agar plates were scraped, serially diluted, spread on TCBS agar for selection of V52, and incubated overnight at 37 °C for enumeration. NC is CFU of V52 alone for 5 hr. ***p < 0.001 vs. V52 CFUs after incubation with PAO1. (B) Overnight-grown P. aeruginosa strains were spot-inoculated on 0.5% agar plate composed of the 0.8% nutrient broth and 0.5% glucose and incubated overnight at 30 °C. (C) CAS assay for iron acquisition activity. The 10-fold diluted supernatants from overnight cultures were reacted with CAS solution for 2 hr, and the OD of the resulting solution was measured at 630 nm. *p < 0.05 vs. iron acquisition activity of PAO1 and Y31.