Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 24;9:568. doi: 10.1038/s41598-018-37071-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Development of three cell co-culture system (MLH) permissive for HCV and HIV replication consisting of macrophages (Mφ), hepatic stellate cells (HSC, LX-2) and hepatocytes (Huh-7). (A) Schematic of co-culture procedure. ‘HS’ denotes for human serum. (B) Huh-7, CFSE-labeled LX-2 and Alexa®647-CD68-labeled Mφ mono-cultures were subjected to FACS analysis. (C) FACS analysis following the MLH co-culture for 9 days. Green and red arrow indicate the detection of CFSE-labeled LX-2 and CD68-labeled Mφ at the end of co-culture. Majority of unlabeled cells belong to Huh-7 cells. (D) Replication of HIV under MLH co-culture for 7 to 8 days with Mφ derived from seven healthy volunteers detected by HIV p24 antigen Elisa assay. (E) Replication of HCV under MLH co-culture condition detected by using HCV core antigen staining.