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. 2019 Jan 24;9:568. doi: 10.1038/s41598-018-37071-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of HCV and/or HIV infection in MLH on αSMA expression in LX2 cells. (A) Normalized gene expression levels of αSMA gene in LX2 cells from seven different MLH co-cultures in the presence or absence of HCV and HIV. (B) FACS analysis of αSMA protein expression profile in LX-2 cells on day 7 of three independent MLH co-cultures with or without HCV and HIV infection. (C) Immunofluorescence analysis of αSMA in LX2 cells on day 7 of MLH co-cultures in the presence or absence of HCV and HIV.