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. 2019 Jan 24;9:737. doi: 10.1038/s41598-018-37402-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gfi1 suppresses the expression of c-Fos, Egr-1 and Egr-2. (A) 32D/Y729F/Gfi1 and FDCP/Y729F/Gfi1 cells were cultured in the absence (Ctr) or presence of Dox for 12 hours. Cells were then starved for 2 hours and stimulated with G-CSF for the indicated times prior to evaluation of c-Fos, Egr-1 and Egr-2 mRNA (A) and protein levels (B).