Fig. 1.

Usp7 promotes the expression of Yorkie (Yki) target genes. All imaginal discs shown in this study were oriented with anterior to the left and ventral up. a, b Late third-instar wing discs of usp7 knockdown (a) or usp7 overexpression (b) were immunostained to show Usp7 (white) and GFP (green). GFP (green) marks the expression pattern of En-gal4 in the wing disc. Of note, mouse anti-Usp7 antibody could recognize Usp7 protein. c-f Wing discs of control (c, e) or expressing usp7 RNA interference (RNAi) by En-gal4 (d, f) were stained to show GFP (green) and diap1-lacZ (white in c, d) or CycE (white in e, f). g, h Wing discs of control (g) or expressing usp7 RNAi by En-gal4 (h) were stained to show Ci (red) or ban-lacZ (white). Ci exclusively expresses in the anterior compartment cells. i-k Wing discs (i, j) or eye disc (k) carrying usp7^KG06814 clones were stained to show the expression of GFP (green) and DIAP1 (white in i), ban-lacZ (white in j) or CycE (white in k). usp7^KG06814 clones are recognized by the lack of GFP. Of note, usp7 mutant cells exhibited decrease of DIAP1 (marked by arrows in i), ban-lacZ (marked by dash line in j), and CycE (marked by arrows in k). l-n Overexpression of usp7 by En-gal4 increased diap1-lacZ (l), CycE (m) and ban-lacZ (n). o, p Wing discs simultaneous expressing usp7 RNAi and Fg-usp7 were stained to show Fg tag (green) and diap1-lacZ (white in o), or ban-lacZ (white in p). Of note, the decreased diap1-lacZ and ban-lacZ caused by usp7 knockdown were restored by the expression of Fg-usp7. q Quantification analyses of indicated signals. Data are means ± SEM. n = 4 biological-independent discs. ^***P < 0.001 by Student’s t-test. Scale bars: 50 μm for all images