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. 2019 Jan 24;10:411. doi: 10.1038/s41467-019-08334-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Usp7 regulates Hippo (Hpo) pathway through Yorkie (Yki). a A wing disc with hpo knockdown was stained to show GFP (green) and CycE (white). b A wing disc simultaneous expressing hpo RNA interference (RNAi) and usp7 RNAi was stained to show GFP (green) and CycE (white). Of note, knockdown of usp7 decreased CycE level in hpo RNAi background. c A wing disc with hpo knockdown was stained to show Ci (red) and diap1-lacZ (white). d Knockdown of usp7 attenuated diap1-lacZ expression in hpo RNAi background. e A wing disc expressing wts RNAi was stained to show GFP (green) and CycE (white). f Knockdown of usp7 decreased CycE protein level under wts RNAi background. g A wing disc expressing wts RNAi was stained to show Ci (red) and diap1-lacZ (white). h Knockdown of usp7 decreased diap1-lacZ expression under wts RNAi background. i Knockdown of yki decreased diap1-lacZ expression. j A wing disc simultaneous expressing yki RNAi and UAS-usp7 was stained to show Ci (red) and diap1-lacZ (white). Of note, overexpression of usp7 could not restore the decreased diap1-lacZ caused by yki knockdown. k A wing disc with yki ectopic expression by En-gal4 was stained to show GFP (green) and CycE (white). Overexpression of yki resulted in CycE upregulation and overgrowth. l Knockdown of usp7 did not rescue the phenotypes induced by yki overexpression. m Knockdown of usp7 decreased Yki protein level. n A wing disc carrying usp7^KG06814 clones were stained to show the expression of GFP (green) and Yki (white). Of note, usp7 mutation resulted in decreased Yki expression (marked by arrows). o A wing disc expressing yki-1w was stained to show Ci (red) and ban-lacZ (white). p A wing disc simultaneous expressing yki-1w and Fg-usp7 was stained to show Fg (green) and ban-lacZ (white). Notably, overexpression of usp7 further elevated ban-lacZ level and resulted in serious overgrowth. Scale bars: 50 μm for all images