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. 2019 Jan 24;10:411. doi: 10.1038/s41467-019-08334-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Usp7 regulates Hippo (Hpo) pathway though its deubiquitinase activity. a, b Wing discs expressing Fg-tagged usp7-ΔMATH were stained to show Fg tag (red) and diap1-lacZ (white in a) or ban-lacZ (white in b). Of note, overexpression of usp7-ΔMATH increased diap1-lacZ and ban-lacZ. c, d Wing discs expressing Fg-usp7-CA were stained to show Fg tag (red) and diap1-lacZ (white in c) or ban-lacZ (white in d). Of note, overexpression of usp7-CA decreased diap1-lacZ and ban-lacZ. e A wing disc simultaneous expressing usp7 RNA interference (RNAi) and Fg-usp7-CA was immunostained to show Fg (red) and ban-lacZ (white). Overexpression of Fg-usp7-CA could not restored the decreased ban-lacZ caused by usp7 knockdown. f Overexpression of Fg-usp7-ΔMATH effectively rescued the attenuation of ban-lacZ induced by usp7 knockdown. g Comparison of adult eye sizes from control flies (g-1), usp7 knockdown (g-2), usp7 overexpression (g-3), usp7-CA overexpression (g-4), and usp7-ΔMATH expression (g-5). Of note, knockdown of usp7 decreased eye size, whereas overexpression of usp7 or usp7-ΔMATH increased eye size. Overexpression of usp7-CA resulted in small eyes. Quantification analyses of the adult eyes were shown on right. h Comparison of adult eye sizes from control flies (h-1), usp7 knockdown (h-2), usp7 overexpression (h-3). Quantification analyses of the adult eyes were shown on right. Data are means ± SEM. n = 10 biological-independent eyes. ^**P < 0.01 and ***P < 0.001 by Student’s t-test. Scale bars: 50 μm for all wing discs (a-f), whereas 200 μm for all images (g, h)