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. 2019 Jan 24;9:582. doi: 10.1038/s41598-018-37335-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regeneration efficiency obtained from non-transformed MB slices of Thompson Seedless, Ciliegiolo, 110 Richter and Kober 5BB: (a) Mean number of shoots per explant. Data were acquired at 3, 6 and 9 weeks of cultures. Data reported are means of ± SE (n = 20). (b) A representative image of the MB slices regeneration of the four genotypes on IM3 medium, kanamycin-free, after three weeks of culture (bar = 1 cm).