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. 2019 Jan 24;9:582. doi: 10.1038/s41598-018-37335-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Percentage of MB slices showing eGFP fluorescence, selected either by the combined use of kanamycin and eGFP screening or by eGFP visual selection alone, after 3, 6 and 9 weeks on culture media. Means with different letters are significantly different according to Newman-Keuls test (p < 0.05) ± SE (n = 50), and compare results obtained from the different genotypes at the same acquisition period. Two independent experiments were performed with similar results. Data reported in the table are related to a unique trial.