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. 2019 Jan 24;9:608. doi: 10.1038/s41598-018-37002-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental model: maternal plasma collection and injection timelines in a CD-1 mouse model of pregnancy. (A) Experimental Design. Plasma from NP mice and pregnant mice was collected on gestation day (E)5, 9, 13, 15, 18, 19, and PP7 for exosome isolation and characterization. (B) Experimental design for PBS and exosome injections to determine the functional role of early (E9), and late gestation (E18) exosomes in vivo. PBS, E9 exosomes, or E18 exosomes were injected every 6 h on E15 and once more on E16. Mice were monitored for preterm labor prior to the expected delivery day (E19).