Fig. 1.

The LIR motif within autophagy receptors is dispensable for Atg8 recruitment during PINK1/Parkin mitophagy. a–f Representative immunofluorescence images of penta KO HeLa cells stably expressing untagged Parkin and either HA-LC3A, HA-LC3B, HA-LC3C, HA-GBRP, HA-GBRPL1 or HA-GBRPL2, without receptor expression (a), or rescued by co-expression of either GFP-OPTN (b), GFP-NDP52 (c), GFP-p62 (d), GFP-OPTN(F178A) (e) or GFP-NDP52(V136S) (f); immunostained for HSP60, HA & GFP after 3 h OA treatment. Atg8-positive mitochondria are indicated by arrowheads. Scale bars: overviews, 10 µm; insets, 2 µm