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. 2019 Jan 24;9:545. doi: 10.1038/s41598-018-36921-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Imaging of tdTomato⁺ single intercalated cell (IC) captured on medium size (10–17 µm) IFC plate. Bulk ICs were sorted from IC reporter (V-ATPase-cre x tdTomato) mice with Flow sorting. Cells were pelleted, counted and suspended in Fluidigm cell suspension reagent at 3:2 ratio at 200 cells/µl concentration. Pooled cells were separated to single IC cells on Fluidigm C1 system and imaged under fluorescence microscope at 20X magnification. (B) Expression of IC markers. Base on the expression patterns of α-IC and β-IC markers, we estimate that cell 1 was a α-IC while cells 2–3 were β-ICs.