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. 2019 Jan 24;9:670. doi: 10.1038/s41598-018-37043-2

Figure 2.

Figure 2

Profile of oxidant levels throughout C. cumulans alimentary canal. (a) Representative gut labelled with DHE or (b) CM-H2DCFDA and analysed under a fluorescence stereomicroscope. Foregut (fg), midgut (mg), mixed segment (ms) and hindgut compartments – p1, p3, p4 and p5. Bars = 1 mm. (c) Fluorescence intensity in arbitrary units (A. U.) of densitometry analysis of DHE labelled guts; ****p = < 0.0001. Fg vs. p1 compartment and fg vs. p4 were also significantly different, p = 0.0045 and 0.0314, respectively. The other combinations presented no significance. The data shown are mean ± S.E.M. from ten replicates with ANOVA and Tukey’s multiple comparisons test.