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. 2019 Jan 24;9:670. doi: 10.1038/s41598-018-37043-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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C. cumulans midgut as an oxidative and mitotically active environment. (a) Midgut labelled with DHE. Analysis under a confocal microscope employing the “z-stack” function and overlap of 350 µm. (b) Midgut mitotic cells revealed by anti-pHH3 antibody (red) and Alexa Fluor 488 phalloidin (green) highlighting muscle fibers. Analysis under a fluorescence stereomicroscope employing the “z-stack” function and overlap of 350 µm. Bars = 100 μm.