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. 2019 Jan 24;9:747. doi: 10.1038/s41598-018-37342-8

Table 1.

Primer for cloning strategy of CerS2-promoter constructs (prom2_I-V) and CerS6 promotor construct (prom6).

Primer Sequence cloning restriction sites Construct size (kbp)
Prom2_I - for TCTAAGTAAGCTTCAGACAGGCTGGAAATCATC HindIII, KpnI/XhoI 1,657
Prom2_I - rev GAATGCCAAGCTTTCCCTCCTTCCTCCTTTG HindIII, KpnI/XhoI 1,657
Prom2_II - for TCTAAGTAAGCTTGTTAGCCAGTGCCCAAAC HindIII, EcoRI 1,977
Prom2_II - rev GAATGCCAAGCTTACCCGCGCACAGACTTACTC HindIII, EcoRI 1,977
Prom2_III - for TATCCATGGGTACCAGAAGTGGGAAACGGAGTAG KpnI 4,779
Prom2_III - rev TATCCATGGGTACCAGCATCCTGAGTGAGGAAAG KpnI 4,779
Prom2_IV - for Restricted from Prom2_III EcoRI 4,079
Prom2_IV - rev Restricted from Prom2_III EcoRI 4,079
Prom2_V - for TATCCATGGGTACCAAATCATGGGCTGAGGTC KpnI 1,180
Prom2_V - rev TATCCATGGGTACCCCTCACAGCGCTTAGAAC KpnI 1,180
Prom6 - for TCTAAGTAAGCTTAGCCAAACAGGTAGTGGAGGGTG HindIII 1,578
Prom6 - rev GAATGCCAAGCTTGCTTTGTCCACTCCGGTG HindIII 1,578