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. 2019 Jan 24;9:641. doi: 10.1038/s41598-018-36385-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Screening of TaCaM4-1 interacting proteins (a) Interaction tests using yeast two-hybrid assays between TaCAM4-1 and prey proteins. Yeasts harboring TaCAM4-1 and prey proteins were placed in different liquid concentrations on control medium SD/-Trp/-Leu and selection medium SD/-Trp/-Leu/-His/-Ade. For negative controls, pGADT7 without insert TaCAM4-1 was used (pGBKT7-TaCAM4-1 + pGADT7). Experiments were performed three times and a representative result is shown. The full-length blots are presented in Supplementary Fig 1. (b) Phylogenetic analyses of TaCAMTA4 and its homologs from different plant species. The TaCAMTA4 protein sequence was used to perform BLAST searches against the National Center for Biotechnology Information database. TaCAMTA4 and its homologs identified in different organisms were aligned. Gm, Glycine max; Vv, Vitis vinifera; At, Arabidopsis thaliana; Sl, Solanum lycopersicum; Zm, Zea mays L; Bd, Brachypodium distachyon; Ta, Triticum aestivum. (c) TaCAMTA4 conserved domains prediction. CG-1, specific CGCG box-containing DNA sequences; TIG, transcription factor ImmunoGlobin; CaM-binding, calmodulin-binding domain; ANK repeat, ankyrin repeats; Bipartite NLS, nuclear locating signal; aa, amino acids.