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. 2019 Jan 24;2:34. doi: 10.1038/s42003-018-0273-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Association of cAMP signaling transition with developmental progression. a Expression of ecmAO::mRFPmars during mound development. Upper panels, DIC images. Lower panels, fluorescent images of ecmAO::mRFPmars. Scale bar, 100 μm. b Time course plot of Flamindo2 (green) and ecmAO::mRFPmars (magenta) signals during mound development. Data were obtained 8–13 h after starvation. The mean intensity of Flamindo2 in a 30 μm² region in the mound shown in a and the mean intensity of ecmAO::mRFPmars in the entire region of the mound were measured. c The sorting of cells expressing ecmAO::mRFPmars at the top of the tight mound. This figure shows trajectories of sorted prestalk cells marked by the expression of ecmAO::mRFPmars at the top of the mound (upper right side of images) during the mound elongation. Scale bars, 100 μm. d Time course plot of inverse Flamindo2 signals during the development of gbfA⁻ cells. Data were obtained 3–10 h after starvation. The mean intensity of Flamindo2 in a 50 μm² region of the cell population was measured. e Wave propagation of Flamindo2 signals in the loose mound of gbfA⁻ cells after 24 h starvation. Images were subtracted at 4 frame intervals to emphasize changes in the signals. Scale bar, 50 μm. f Time course plot of inverse Flamindo2 signals in the mound shown in e. The mean intensity of Flamindo2 in a 20 μm² region in the mound shown in e was measured