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. 2019 Jan 24;9:507. doi: 10.1038/s41598-018-36598-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Temporal evolution of the tissue-repair cytokine Transforming Growth Factor β1 (TGFβ1) after 30 minutes MCAO. (a) Low magnification images from ipsilateral to lesion striatal tissue sections taken at different time-points after ischemia and immunostained for TGFβ1 (red). A dashed line indicates the lesion boundary (L). Scale bar: 50 μm. Sections were counterstained for reactive astrocytes ((b), GFAP staining in green) or microglia ((c), Iba1 staining in green) and shown at higher magnification. Arrows (astrocytes) or arrohweads (microglia) point towards cells that express TGFβ1. DAPI (blue) was used as a nuclear counterstaining. The images shown are representative of at least three animals per time-point. Scale bars: 20 μm.