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. 2019 Jan 24;9:597. doi: 10.1038/s41598-018-37160-y

Figure 11.

Figure 11

Assay to image the phagocytosis of apoptotic human red blood cells. (A) Brightfield images of control and A23187-treated human red blood cells, and labeling of phosphatidylserine with Alexa Fluor 594-conjugated annexin V (AF594-Annexin V; red channel). Scale bar: 10 µm. (B) Introduction of human red blood cells (hRBCs) with externalized phosphatidylserine (PS) to wild-type (WT) mouse peritoneal macrophages (MΦs). Scale bar: 10 µm. (C and D) Time-lapse images (XZ and reconstructed 3D views) of macrophages ingesting the three hRBCs (labeled 1,2 and 3, respectively) shown in the brightfield image of panel B.