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. 2019 Jan 24;9:597. doi: 10.1038/s41598-018-37160-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ingestion of apoptotic human red blood cells by a Myo10^tm2/tm2 macrophage. (A) Introduction of human red blood cells (hRBCs) with externalized phosphatidylserine (PS) to a Myo10^tm2/tm2 peritoneal macrophage (MΦ). Scale bar: 10 µm. (B and C) Time-lapse images (XZ and reconstructed 3D views) of a Myo10^tm2/tm2 macrophage ingesting the two hRBCs (labeled 1 and 2, respectively) shown in the brightfield image of panel A. (D) Time-lapse brightfield images showing two human red blood cells being packed together after their respective phagosomes, shown in the insets of panel C, have fused. (E) Summary data for wild-type (WT; n = 3) versus Myo10^tm2/tm2 (n = 2) mice. n.s. = not significant.