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. 2019 Jan 24;9:597. doi: 10.1038/s41598-018-37160-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Headless mouse Myo10 (Hdl-mMyo10) strongly localizes to the cell periphery independent of the MyTH4-FERM domain. (A) Schematic representations of the domain structures of N-terminal EGFP-tagged mouse Myo10 (EGFP-mMyo10), Hdl-mMyo10 (EGFP- Hdl-mMyo10) and truncated variants thereof. (B) Images of fixed HEK293T cells after transfection with EGFP-mMyo10 or various EGFP-tagged Hdl-mMyo10 constructs. Cells were counterstained with Alexa Fluor 594-conjugated phalloidin, a red fluorescent F-actin probe, and imaged via a Zeiss Plan Apo 63/1.4 (oil-immersion) objective lens by superresolution structured illumination microscopy. Scale bars: 10 µm.