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. 2019 Jan 24;9:597. doi: 10.1038/s41598-018-37160-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of headless Myo10 to the plasma membrane requires pleckstrin homology (PH) domains. (A) Fluorescence images of living HEK293T cells transfected with EGFP-tagged, full-length mouse Myo10 (mMyo10-EGFP) or various EGFP-tagged headless mouse Myo10 (Hdl-mMyo10) constructs. Cells were counterstained with the red fluorescent plasma membrane marker CellMask Orange. Images were obtained by spinning disk confocal microscopy via a Nikon Apochromat TIRF 60x/1.49 (oil-immersion) objective lens. Scale bars: 10 µm. Plots of gray value intensity for the superimposed lines (dark yellow lines in the middle column) are shown on the right. In the plots of intensity along the lines, peaks of the CellMask Orange (red) traces serve as plasma membrane markers. (B) Profile data quantification. Plot of relative plasma membrane localization, indexed as the ratio of the maximum of the plasma membrane (PM) peak to mean cytosolic intensity.