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. 2019 Jan 24;9:597. doi: 10.1038/s41598-018-37160-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Myo10 expression in retinal vascular endothelial cells and retinal vascularization in Myo10^tm2/tm2 mice. (A) Expression profile of the myosin superfamily in postnatal retinal endothelial cells. Notably, RNA sequence analysis revealed increased expression of Myo10 between postnatal day 6 (P6) and P15 (n = 3 independent preparations per group). (B) Schematic diagram showing vascularization of the deeper layers of the retina between P7 and P15. (C) Whole-mount retinas from wild-type (WT) and Myo10^tm2/tm2 mice stained for blood vessels (representative of 6 preparations for each genotype). The white arrow indicates persistent hyaloid vessels. Quantitative analysis of the vascularization of WT versus Myo10^tm2/tm2 retinas is shown on the right (n = 6 retinal preparations (from 3 mice) for each group). Vascular area is the percentage of area covered by the vascular network and lacunarity is a measure of heterogeneity. Scale bars: 1 mm. (D) Higher magnification images of isolectin B4-stained retinal blood vessels. Quantitative analysis of vessel branching (branch points/unit area) is shown on the right. Scale bars: 500 µm. (E) Higher magnification images (obtained using a 20x/0.45 objective lens) of retinal vascular layers, color coded blue (deep plexus), green (intermediate (inter.) plexus) and red (superficial (superf.) plexus). Scale bars: 50 µm. (F) Side-view projections of 3D reconstructions of retinal vessels, obtained by spinning disk confocal microscopy (via a 20x/0.45 objective lens). Anti-collagen, type IV, stained the superficial plexus (red), whereas all vessels were labeled with isolectin B4 (green). Scale bars: 100 µm.