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. 2019 Jan 24;9:653. doi: 10.1038/s41598-018-37441-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fine epitope mapping of 108-B6 and 4A68 antibodies. (a) Schematic diagram of recombinant PGRMC1 fragments (residues 1–25, 1–43, 1–95, 1–157, 1–170, 1–182 and 1–195) used in this study. (b) A series of depletion mutants of PGRMC1 gene were synthesized and separated by agarose gel electrophoresis. The deletion mutants of PGRMC1 genes were detected by ethidium bromide staining. (c) Individual fusion proteins were expressed in E. coli DH5α as fusion proteins with GST tag at the N-terminus, and stained with CBB R-250 after SDS-PAGE. (d,f) Western blot analysis of GST-PGRMC1 fusion proteins with anti-GST (d), 4A68 (e), and 108-B6 (f) antibodies. The asterisks indicate partial degradation products of GST-PGRMC1 fusion proteins.