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. 2019 Jan 24;9:598. doi: 10.1038/s41598-018-36959-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AFM vertical deflection images depicting the internal structure of primary and fibrous shell portions of Magellania venosa. Corresponding lateral deflection images are shown in Fig. A2. (a) Close-up of the primary layer and the first three rows of adjacent fibres visualizing the gradual changeover from primary to fibrous calcite shell layers. (b) Biopolymer membrane tightly attached to the calcite of a fibre along its proximal, convex surface. The organic membrane (black stars) is between two adjacent fibres (red and white stars) and in each case the biopolymer lines the basal (proximal), convex portion of the fibre.