Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 24;9:455. doi: 10.1038/s41598-018-37034-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of proximity channeling on GOx-HRP cascade. (a) A model of a GOx-HRP complex and the distribution of hydrogen peroxide (H₂O₂, intermediate of the GOx-HRP cascade) shown as a deviation from the bulk concentration, Δ[H₂O₂] = [H₂O₂] − [H₂O₂]_bulk. (b) Enhancement v_ch/v_non due to proximity channeling as a function of the bulk concentration of hydrogen peroxide. Symbols show the results of the full numerical calculations and the lines have been obtained using equation (1). (c) Reaction velocities of the channeled (symbols) and non-channeled (lines) reactions as functions of concentration of catalase (enzyme competing with HRP for hydrogen peroxide). (d) Channeling diagram showing the region where the enzyme proximity can at least double the reaction velocity. The diagram is drawn in the plane of the concentration of enzyme complexes and the concentration of catalase. We have assumed that [GOx-HRP] is the same as [GOx] = [HRP] in the non-channeled system. Squares show the results of the full numerical calculations and the line has been obtained using equation (3). The diamond shows the system with the maximal concentration of catalase studied experimentally by Zhang et al.³⁴.