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. 2019 Jan 24;9:669. doi: 10.1038/s41598-018-36724-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of DN EGFR diminishes hematogenous invasion of the eye and brain by T. gondii. (a) Dendritic cells infected in vitro with PTG T. gondii were injected i.v. to Trg-Ctr or Trg-DN EGFR mice. Retinas and brains were obtained 1 d after i.v. challenge. Expression of B1 gene was assessed by qPCR. Levels were compared to those of one Trg-Ctr mouse that was given an arbitrary value of 1. (b) Mice were injected i.v. with 5 × 10⁵ PTG tachyzoites. T. gondii B1 gene was examined 1 day post-challenge and assessed as above. 8–12 mice per group pooled from 2–3 independent experiments. **p < 0.01; ***p < 0.001 (Student’s t test).