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. 2019 Jan 24;9:591. doi: 10.1038/s41598-018-37827-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histochemical analysis of cell wall reinforcement in the leaves and roots of soybean at 24 h post-inoculation. (a,f) Control, (b,g) NBC001 treatment, (c,h) NBC001 + J2 treatment, and (d,i) J2 treatment. Scale bars represent 500 μm. (e,j) Callose deposits were quantified using ImagePro 5.0 software. The values are the mean callose deposits area ± standard deviation from 20 pieces of leaves (diameter, 1 cm) or the roots from five plants.