Table 2.
EPS assessment in relation to bacterial and fungal growth.
| Bacterial population log CFU/ml1 | EPS titre (µg/ml) | EPS D-GA content (%) | D-GA (µg/ml) | F. graminearum DNA titre (ng/µl)2 | |
|---|---|---|---|---|---|
| Control | 1.19 ± 0.11a1* | 0.8 ± 0.16a | 6 | 0.05 ± 0.01a | 13 ± 2.6 |
| Wheat spike wash | |||||
| Wheat spike wash A26 (Low)3 | 3.12 ± 0.12b | 10 ± 2b | 3 | 0.3 ± 0.06b | <0.3 |
| A26 (High) | 3.09 ± 0.13b | 10 ± 1.5b | 3 | 0.3 ± 0.03b | <0.3 |
| Wheat spike wash A26Sfp (Low) | 3.02 ± 0.10b | 14 ± 2.6c | 3.5 | 0.5 ± 0.1c | 4.5 ± 0.8 |
| A26Sfp (High) | 3.02 ± 0.09b | 15 ± 2.4c | 6 | 0.9 ± 0.05d | <0.3 |
| ½ TSB cultures | |||||
| ½ TSB cultures A26 | 8.89 ± 0.16 | 10.05 ± 2.23 | 3 | 0.3 ± 0.05 | ND |
| A26Sfp | 8.69 ± 0.11 | 15.02 ± 2.57 | 6 | 0.9 ± 0.09 | ND |
| Sodium alginate | 15 | 82 | 8.3 ± 0.83 | ND | |
| Levan | 15 | 0 | ND | ND | |
1The bacteria were re-isolated by selective plating and confirmed by PCR13. 1*Counting of bacterial colony forming units was performed on ½ TSB plates
2F. graminearum DNA was isolated amplified and quantified using the Invitrogen™ Qubit™ 4 Fluorometer as described in Material and Methods.
3Two inoculum densities of A26 and A26Sfp (Low) −106 and (High) 108 cells per ml. Different letters indicate statistically significant differences (p < 0.05).
ND- not detected.