Fig. 3.

IRE1/XBP1s pathway is required for multiple hallmark pathways in PCa. LNCaP cells were either left untreated or subjected to siRNA-mediated XBP1 knockdown. In parallel, cells were either treated with vehicle or MKC8866. Total RNA was isolated and used in NextGen sequencing. a The presence of the XBP1s junction is measured in the spliced RNA-seq reads and represented as a ratio of total spliced reads. The box represents the interquartile range, the horizontal line in box is the median, and the whiskers represent 1.5 times interquartile range. b The expression levels of XBP1s, XBP1u, and three XBP1s target genes were determined by qPCR. *P < 0.05, Student t-test, error bars denote SD. c Scatter plot representing the concordance of gene expression data obtained upon XBP1 knockdown and MKC8866 treatment. d Hallmark pathways enriched under both MKC8866-treated and XBP1 knockdown cells. Graph displays category scores as −log₁₀ (P value) from Fisher’s exact test. e Hallmark pathways MYC TARGETS V1 and V2 both enriched by GSEA on combined data of siXBP1 and MKC8866 treatment