Fig. 1.

Dynamic RT-DC. a Sketch of experimental setup showing microfluidic channel with translocating cell and corresponding region-of-interests at selected time points t₁, t₂, t₃, and t_n. b Time series of a suspended cell while translocating a 30 µm × 30 µm channel of 300 µm length. Length of the scale bar is 10 μm. c Deformation (1-circularity) trace of 92 single cell measurements along axial position z in the channel (blue dots). The yellow and green line originate from an exponential fit characterizing two apparent cell relaxation modes (top). The red vertical lines indicate channel inlet and outlet position. Finite element method (FEM) calculations of hydrodynamic shear stress profile along the same microfluidic channel axis at a flow rate of 8 nl s⁻¹ in the absence of cells (bottom). Insets show velocity and stress distribution of the channel cross-section