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. 2019 Jan 24;9:500. doi: 10.1038/s41598-018-36735-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expansion of primary NHBE cells in M3 (PnCEx-PLUS) expansion medium. Expansion of NHBE cells from three different donors was achieved using M3 expansion medium. (A) Primary NHBE cells could be expanded up to eight passages before growth declined. (B) Population doublings were fairly consistent up to the final passage number. Primary NHBE were expanded 8 times within 30 days for most donors. ^ΨCells at this passage failed to reach 70–80% confluency within 7 days.