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. 2019 Jan 24;9:500. doi: 10.1038/s41598-018-36735-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of passage number on phenotypic characteristics including ASL, CBF, and cilia length. NHBE 3D culture characteristics were measured upon reaching 4 weeks at ALI. (A) ASL of 3D NHBE passaged cultures. (B) CBF of NHBE passaged 3D cultures, expressed in beats/second. (C) Cilia length (µm) of NHBE passaged 3D cultures. Data collated from 3 different donors, n = 3 for each donor. Significance compared to Passage 1, ****p < 0.0001.