Table 2.

List of the different models, their variation compared to the previous model, and the outcome or idea for the next model

Model no.	Variation compared to previous model	Features/limitations	Required improvements
01	Basic model	Strong HP deflection at day/night transition	Better map day/night transition
02	Adjusted starch synthesis and degradation (r3 and r4)	Sharp deviance of CaAa pool at day/night transition	Introduce a constant respiration rate
03	Constant respiration rate during day and night		Split carbon pools to converge closer to the in vivo condition
04	Split carbon pool into a citrate and combined malate/fumarate pool	increased dynamics for Cit and MalFum	Interconversion of Cit and MalFum as in the TCA cycle
05	Interconversion between Cit and MalFum	Long simulation times (up to 8 h per simulation)	Splining of enzyme parameters to reduce simulation time
06	Splines for Vm values for sucrose cycling enzymes	Flat dynamics for Glc and Frc	Splining of selected enzyme parameters
07	Spline for SPS and Inv	Shorter simulation times (up to 4 h per simulation)	Exact distribution of excess carbon to shoot or root
08	Implementing the calculated SC gain	Final model

HP hexose phosphates, CaAa carbonic- and amino acids, Cit citrate, MalFum malate and fumarate pool, Glc glucose, Frc fructose, SPS sucrose-phosphate-synthase, Inv invertase, SC structural carbon