Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 24;9:485. doi: 10.1038/s41598-018-37284-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Gene expression data cluster according to developmental time point and confirm successful Purkinje cell (PC) capture. (A) Schematic diagram of mouse PC development from postnatal day 0 (P0) until P21. This developmental period is characterized by extensive morphological changes and the formation of synaptic connections with climbing fibers (CF), Basket cells (BC) and parallel fibers (PF) of cerebellar granule neurons (CGN). BG: Bergmann glia; EGL: external granular layer; GC: Golgi cells; IGL: internal granular layer; ML: molecular layer; PCL: Purkinje cell layer; SC: Stellate cells. (B) PCs were effectively captured at independent developmental stages. Scatter plot of the first two principal components (PC1/2) for all samples, displaying significant groupings according to postnatal day. 95% confidence ellipses for these groups are shaded in grey, displaying full separation. (C) Predominant capture of PC-specific transcripts. Gene expression data (RPKM values) across developmental time points (X-axis) for two cell-specific markers for each cell-type. CGN: cerebellar granule neurons; B/SC: basket/stellate cells; GC: Golgi cells; BG: Bergmann glia. See also Supplementary Fig. S1.