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. 2019 Jan 24;9:447. doi: 10.1038/s41598-018-36940-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Huaier n-butanol extract inhibited the colony formation of GC cells. (A) Representative images of cell colonies after treatment with various concentrations of Huaier n-butanol extract. The colony formation of MGC803 and HGC27 cells was significantly reduced with increasing concentrations of Huaier n-butanol extract treatment. (B) The colony formation rate after treatment with Huaier n-butanol extract was calculated as the percentage of area covered by colonies per view (n = 3, bar graphs are plotted as the mean ± SD, **p < 0.01, ***p < 0.001, Student’s t-test).