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. 2019 Jan 24;9:447. doi: 10.1038/s41598-018-36940-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Huaier n-butanol extract induced cell cycle arrest in GC cells. (A) MGC803 and HGC27 cells were treated with gradient concentrations of Huaier n-butanol extract. Fluorescence-activated cell sorting analysis showed that cells accumulated in S and G2/M phases. (B) Quantitative analysis of cell cycle distribution. Cell cycle distribution of MGC803 and HGC27 cells was analysed. Data are presented as the mean ± SD of three independent experiments. (C) Effects of Huaier n-butanol extract on the expression of p21 and cyclin D1 in GC cells. HGC27 GC cells were treated with 0, 40, and 80 μg/ml of Huaier n-butanol extract for 48 h, and cell lysates were subjected to western blot analysis with p21 and cyclin D1 antibodies.