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. 2019 Jan 24;9:447. doi: 10.1038/s41598-018-36940-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of Bmi1 reversed the effects of Huaier n-butanol extract on GC cells. (A) MGC803 GC cells were transfected with a Bmi1 plasmid for 48 h. Cell lysates were subjected to immunoblot analysis to examine the expression of Bmi1. (B) Proliferation of MGC803 cells overexpressing Bmi1 or the negative control. (C) MGC803 cells transfected with the negative control or the Bmi1 plasmid were subjected to wound healing assays and observed at 0 and 48 h. (D) Quantitative analysis of the migration speed using the migration index (n = 3, bar graphs are plotted as the mean ± SD, **p < 0.01, Student’s t-test). (E) MGC803 cells transfected with the negative control or the Bmi1 plasmid were subjected to transwell assays. White bars represent 100 μm. (F) Quantitative analysis of invaded cells (n = 3, bar graphs are plotted as the mean ± SD, **p < 0.01, Student’s t-test).