Figure 4.
Assay of seed germination rate of GhWRKY33 overexpression transgenic Arabidopsis with mannitol treatment. (A) Quantitative RT-PCR analysis of GhWRKY33 expression in the transgenic lines and wild type. (B–E) Statistical analysis of seed germination rate. Seeds of the transgenic lines and wild type were germinated on MS medium (B), MS medium with 200 mM mannitol (C), MS medium with 250 mM mannitol (D), and MS medium with 300 mM mannitol (E). Each curve represents an average of three replicates. WT, wild type; L2, L3 and L4, three GhWRKY33 overexpression transgenic lines.
