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. 2019 Jan 24;9:724. doi: 10.1038/s41598-018-37035-2

Figure 7.

Figure 7

Assay of GhWRKY33 overexpression transgenic Arabidopsis tolerant to ABA. (AC) Seed germination rates of GhWRKY33 transgenic lines and wild type with different ABA concentrations (0, 2 and 5 μM). Germination was recorded daily. (DF) Seedlings of transgenic lines and wild type with 0, 10 and 15 μM ABA treatment. (GI) Statistical analysis of the root length of seedlings with 0, 10 and 15 μM ABA treatment. (J) Micrographs of stomatal changes of the transgenic lines and wild type with or without 20 μM ABA treatment. (K) Measurement of statistical analysis of stomatal closure (stomatal length:width ratio) of the transgenic lines and wild type with or without ABA treatment (n > 40 for each sample). Mean values and standard errors (bars) are shown from three independent experiments. One and two asterisks represent there was a significant difference (P < 0.05) and a very significant difference (P < 0.01) in Duncan’s multiple range test, respectively. CK, seedlings grew under normal conditions as controls; ABA, seedlings grew with 20 μM ABA treatment; WT, wild type; L2, L3 and L4, three GhWRKY33 overexpression transgenic lines.