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. 2019 Jan 24;9:651. doi: 10.1038/s41598-018-36912-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Neuronal activity enhances the expression of ARC and c-Fos proteins DIV55 neurons were incubated overnight with TTX, thereafter with KCl for up to 4hrs and assessed by Western blot for c-Fos and ARC proteins. Note the ATM-independent induction of these IEGs, as also evident from the WT1 treated with ATMi (right panel). Vinculin and β-Actin are loading controls for proteins.