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. 2018 Jun 28;176(4):671–683. doi: 10.1111/bph.14372

Figure 2.

Figure 2

(A) Selected region for stacked 1H‐NMR spectra of 20 mM 2HED + 10 mM NaHS with increasing additions of IAM (1–5 mM, bottom to top spectrum respectively). Increasing additions of IAM causes subsequent decreases in the concentration of 2ME (δ = 2.67–2.70 and 3.68–3.72 ppm) and 2HET (δ = 3.08–3.11 and 3.91–3.94 ppm) because IAM competes with 2HED for the trapping of the intermediate 2ME‐SSH. (B) Plot for (2HET) as a function of increasing concentrations of exogenous electrophiles IAM (black squares) or NEM (black diamonds) (0–5 mM). Increasing addition of both electrophiles causes a subsequent decrease in the concentration of 2HET formed. All 1H‐NMR experiments were performed in 100 mM phosphate buffer (pH 7.4) with 10% D2O and 1 mM DSS as an internal reference.