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. 2019 Jan 24;9:526. doi: 10.1038/s41598-018-36717-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Anti-GABARAP (8H5) antibody detects YFP-GABARAP, but not YFP-GABARAPL1 and CFP-GABARAPL2 in immunofluorescence. HAP1 GABARAP KO cells were transfected with YFP-GABARAP and YFP-empty vector (A) or YFP-GABARAPL1 and CFP-GABARAPL2, respectively (B). After 48 h cells were incubated for 3 h with 100 nM Bafilomycin A1 (BafA1) in growth factor depleted medium (w/o FCS). Fixed cells (4% PFA) were immunolabeled with anti-GABARAP (8H5) antibody. Colocalization of GABARAP (green) and YFP (red) is indicated by yellow puncta. Nuclei were counterstained with DAPI.